کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2833487 1163873 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells
چکیده انگلیسی

RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 44, Issue 4, January 2007, Pages 521–529
نویسندگان
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