کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2833563 1163877 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mass spectrometry-based relative quantification of human neutrophil peptides 1, 2, and 3 from biological samples
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Mass spectrometry-based relative quantification of human neutrophil peptides 1, 2, and 3 from biological samples
چکیده انگلیسی

Human neutrophil peptides (HNPs) are cysteine-rich antimicrobial peptides stored in neutrophils. The similar structure of HNPs -1, -2, and -3 renders them impossible to study individually in biological samples. For the first time, we describe a method of individually identifying the HNPs -1–3 from exudative neutrophils using matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry, and we demonstrate the ability to quantify the relative changes in the peptides found in biological samples.The study includes tracheal aspirates (TA) from infants with respiratory syncytial virus (RSV) illness at intubation for respiratory failure (acute illness) and at extubation (convalescence). In vitro, convalescent and acute illness TAs are labeled with d0- and d3-acrylamides, respectively, and mixed 1:1. TA proteins are separated by one-dimensional gel electrophoresis and then identified by mass spectrometry-based peptide mass fingerprinting. The ratio of signal intensities for the isotopically normal (d0-labeled) and heavy (d3-labeled) forms of the peptide reveals the relative increase in each peptide with illness.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 43, Issue 9, March 2006, Pages 1485–1489
نویسندگان
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