کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2833973 1164275 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phylogenetic analysis of the Argonaute protein family in platyhelminths
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Phylogenetic analysis of the Argonaute protein family in platyhelminths
چکیده انگلیسی

Argonaute proteins (AGOs) are mediators of gene silencing via recruitment of small regulatory RNAs to induce translational regression or degradation of targeted molecules. Platyhelminths have been reported to express microRNAs but the diversity of AGOs in the phylum has not been explored. Phylogenetic relationships of members of this protein family were studied using data from six platyhelminth genomes. Phylogenetic analysis showed that all cestode and trematode AGOs, along with some triclad planarian AGOs, were grouped into the Ago subfamily and its novel sister clade, here referred to as Cluster 1. These were very distant from Piwi and Class 3 subfamilies. By contrast, a number of planarian Piwi-like AGOs formed a novel sister clade to the Piwi subfamily. Extensive sequence searching revealed the presence of an additional locus for AGO2 in the cestode Echinococcus granulosus and exon expansion in this species and E. multilocularis. The current study suggests the absence of the Piwi subfamily and Class 3 AGOs in cestodes and trematodes and the Piwi-like AGO expansion in a free-living triclad planarian and the occurrence of exon expansion prior to or during the evolution of the most-recent common ancestor of the Echinococcus species studied.

Figure optionsDownload as PowerPoint slideHighlights
► A subclass of AGOs exclusive to platyhelminths.
► Loss of Piwi-like AGOs in trematodes and cestodes.
► Expansion of Piwi-like AGOs in free-living planarian.
► Exon expansion before speciation of two Echinococcus parasites.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Phylogenetics and Evolution - Volume 66, Issue 3, March 2013, Pages 1050–1054
نویسندگان
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