کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836266 | 1570848 | 2015 | 7 صفحه PDF | دانلود رایگان |
• AY-WB phytoplasma gene expression in plants and insects was analysed by qRT-PCR.
• 74 and 34 genes were up-regulated in insects and plants, respectively.
• Stress response and metabolism-related genes were up-regulated in insects.
• Membrane-associated proteins genes were up-regulated in plants.
Phytoplasmas are intracellular insect-transmitted phytopathogenic bacteria with small genomes. To understand how Aster Yellows phytoplasma strain witches' broom (AY-WB) adapts to their hosts, we performed qRT-PCR analysis of 179 in silico functionally annotated AY-WB genes that are likely to have a role in host adaptation. 74 genes were up-regulated in insects and included genes involved in stress response, phospholipid synthesis, malate and pyruvate metabolism, hemolysin and transporter genes, multiple copies of thymidylate kinase, sigma factor and Zn-proteases genes. In plants, 34 genes encoding an immune dominant membrane protein, membrane-associated proteins, and multidrug resistance ABC-type transporters, were up-regulated. Differential regulation of gene expression thus appears to play an important role in host adaptation of phytoplasmas.
Journal: Physiological and Molecular Plant Pathology - Volume 91, July 2015, Pages 81–87