کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836330 | 1570859 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identifying cotton (Gossypium hirsutum L.) genes induced in response to Aspergillus flavus infection Identifying cotton (Gossypium hirsutum L.) genes induced in response to Aspergillus flavus infection](/preview/png/2836330.png)
Development of aflatoxin-resistant cotton against Aspergillus flavus is handicapped by the lack of resistance source in available germplasm. Genetic engineering warrants for identification of resistance-associated genes in cotton. As a first step toward this, we isolated 44 differentially expressed genes (DEGs) in response to A. flavus infection, using annealing control primer system. Different functional classification of the DEGs suggested a complex and multi-factorial plant–fungus interaction. Eight DEGs, including transcription factors, kinase, and downstream stress responsive genes, showed a tissue- and time-dependent differences in their expression. The upregulated genes can be used as transgenes and/or functional markers for breeding aflatoxin-resistant cottonseed.
► Cotton genes involved in the primary stage of Aspergillus flavus infection.
► Transcription factors and protein kinases as key candidates.
► Annealing control primer system to identify differentially expressed genes in cotton.
► Cross-talk between A. flavus infection and other abiotic stresses.
Journal: Physiological and Molecular Plant Pathology - Volume 80, October 2012, Pages 35–40