کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836609 | 1570867 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Time RT-PCR. In in vitro cultures, the α-cinnamomin gene showed the highest level of expression, the β-cinnamomin gene (β-cin) was the most inducible, and the HAE transcripts were in low abundance. Transcription of all the elicitins was active during the active growth of the pathogen when infecting cork oak (Quercus suber) roots, and as host colonization progressed, the level of β-cin expression fell, while that of α-cin rose. In an antisense transgenic strain, the silencing of β-cin also negatively affected the expression of other elicitin genes in the cluster. The reduced in planta growth of the β-cin knock-out is related to the altered pattern of elicitin gene expression, supporting the idea that one of the functions of elicitins is related, directly or indirectly, with pathogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 73, Issues 1â3, October 2008, Pages 48-57
Journal: Physiological and Molecular Plant Pathology - Volume 73, Issues 1â3, October 2008, Pages 48-57
نویسندگان
MarÃlia Horta, Nelson Sousa, Ana C. Coelho, Dina Neves, Alfredo Cravador,