Proteome changes in sugar beet in response to Beet necrotic yellow vein virus

Beet necrotic yellow vein virus (BNYVV) is a devastating sugar beet pathogen. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective disease control strategies will come from a better understanding of disease and resistance. BNYVV-induced differential sugar beet protein expression was evaluated with multidimensional liquid chromatography. Of more than 1000 protein peaks detected in root extracts, 7.4 and 11% were affected by BNYVV in the resistant and susceptible genotypes, respectively. Using tandem MALDI-TOF-MS, 65 proteins were identified; differential expression of some was verified using RT-PCR. Proteomic data suggest involvement of systemic resistance components in Rz1-mediated resistance and phytohormones in symptom development.