
Keywords: GST، گلوتاتیون S-ترانسفراز; Heparin; Affinity tag; Single-step; Recombinant proteins and protein purificationHB, heparin-binding; GST, glutathione-S-transferase; GSH, reduced glutathione; IPTG, isopropyl thiogalactopyranoside; PMSF, phenyl methyl sulfonyl fluoride