کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836766 | 1570874 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of RB-orthologous genes from the late blight resistant wild potato species Solanum verrucosum
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A key long-term management strategy for combating this disease is to develop potato cultivars with high levels of durable resistance through identification and integration of major resistance genes. The RB gene, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers broad-spectrum resistance to potato late blight. Here, we have determined the late blight resistance phenotypes of eight accessions of Solanum verrucosum, another wild diploid potato species, using greenhouse inoculations and discovered variability among the accessions. While most accessions were resistant, one accession was notably more susceptible. Transcribed orthologs of the RB gene from the eight S. verrucosum accessions were cloned using a homology-based PCR approach. Sequence analysis revealed that the RBver orthologs share up to 83.5% nucleotide identity with RBblb. Stable introduction of the RB ortholog from late blight resistant S. verrucosum PI275260 into susceptible S. tuberosum confers resistance to P. infestans. Interestingly, this functional RBver ortholog contains an insertion of a complete leucine rich repeat when compared to RBblb, and differs from the RBver ortholog from a susceptible accession at only four amino acid residues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 69, Issues 4â6, OctoberâDecember 2006, Pages 230-239
Journal: Physiological and Molecular Plant Pathology - Volume 69, Issues 4â6, OctoberâDecember 2006, Pages 230-239
نویسندگان
Zhenyu Liu, Dennis Halterman,