کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2840409 | 1570995 | 2015 | 10 صفحه PDF | دانلود رایگان |
• Phylogenetic analyses of insect Drosha, Dicer-1 and Argonaute-1 proteins.
• Wide spread tissue transcript distribution of drosha, dicer-1 and argonaute-1 in adult desert locusts.
• Rapid upregulation of drosha and argonaute-1 during locust phase transition.
• Rapid downregulation of drosha, dicer-1 and argonaute-1 following feeding of starved locusts.
In this article, we identify and characterise the miRNA machinery components Drosha, Dicer-1 and Argonaute-1 of the desert locust. By means of phylogenetic analyses, we reveal important insights in the evolutionary context of these components. Our data illustrate that insect Argonaute-1 proteins form a monophyletic group with ALG-1 and ALG-2 of Caenorhabditis elegans and with the four (non-Piwi) Argonaute proteins present in humans. On the other hand, humans apparently lack clear homologues of the insect Argonaute-2 proteins. In addition, we demonstrate that drosha, dicer-1 and argonaute-1 display wide transcript tissue-distribution in adult desert locusts, and that during locust phase transition and feeding of starved locusts the expression levels of the miRNA pathway are regulated at the transcript level.
Figure optionsDownload as PowerPoint slide
Journal: Journal of Insect Physiology - Volume 75, April 2015, Pages 20–29