کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2843477 1166098 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation on supraphysiological thermal injury in two well-differentiated human hepatoma cell lines, HepG2 and Hep3B
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Investigation on supraphysiological thermal injury in two well-differentiated human hepatoma cell lines, HepG2 and Hep3B
چکیده انگلیسی

Hyperthermia is a promising treatment for carcinoma cells. The thermal injuries of two hepatoma carcinoma cell lines with the identical cytological grade, HepG2 and Hep3B cell lines, were investigated systematically in the present study. The homemade heating stage was used to provide a constant temperature between 40 and 70 °C for thermal treatment. When the cells were exposed to temperatures ranging from 40 to 45 °C, Hep3B cells had a lower thermotolerance than the HepG2 cells; however, the survival rate of these two cell lines was still high. The differences in thermotolerance between HepG2 and Hep3B cells were more significant at the range of 50–55 °C than those at lower-level temperatures of 40–45 °C. Furthermore, the viability of the cells was less than 10% when they were exposed to a supraphysiological temperature of 60 °C for 5 min; these cell lines suffered from injury saturation under that thermal treatment. The statistical analysis also concluded that Hep3B cells are more susceptible to heat stress than are the HepG2 cells when subjected to the thermal treatment applied in this work, the exception being when thermal injury saturation occurred. The kinematic parameters of the activation energy and frequency factor for HepG2 and Hep3B cells were also quantitatively determined herein. The activation energies (ΔE) for HepG2 and Hep3B cells were 170.17 and 152.44 kJ/mol, respectively. Furthermore, the frequency factors (A) for HepG2 and Hep3B cells were 4.11×1024 and 1.07×1022 s−1, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Thermal Biology - Volume 35, Issue 8, December 2010, Pages 411–416
نویسندگان
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