کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2842653 1571088 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Temperature induced modulation of lipid oxidation and lipid accumulation in palmitate-mediated 3T3-L1 adipocytes and 3T3-L1 adipocytes
ترجمه فارسی عنوان
مدولاسیون دما ناشی از اکسیداسیون چربی و تجمع چربی در سلولهای چربی پالمیتات واسطه 3T3-L1 و سلولهای چربی 3T3-L1
کلمات کلیدی
درجه حرارت؛ آديپوسيت ها؛ استرس اکسیداتیو؛ اندازه قطره لیپید؛ زنده ماندن سلولها
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• We explore temperature induced modulation of cell metabolism in palmitate-mediated 3T3-L1 adipocytes and 3T3-L1 adipocytes.
• Cooler temperatures modulated oxidative stress to improve the physiological fusion of lipid droplets and cell viability.
• Temperature reductions regulate lipid oxidation and lipid size, but can not remedy the palmitate damage induced cell death.

Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid accumulation in 3T3-L1 adipocytes and palmitate-treated adipocytes after 4 h incubation. No significant differences of viability in 3T3-L1 adipocytes were detected under different temperature conditions. Despite no significant increase being observed under warm temperature (39 °C) conditions, a similarly significant suppression of intracellular reactive oxygen species (ROS) and lipid peroxidation were found in 3T3-L1 adipocytes and palmitate-treated adipocytes under 4 h exposure to cooler temperatures of 31–33 °C (P<0.01). ROS, chemically reactive molecules containing oxygen, are currently understood to be a major contributor to oxidantive stress in obesity. Additionally, cooler temperatures (31–33 °C) could improve the size of lipid droplets in 3T3-L1 adipocytes (P<0.01), but no significant effect was generated by temperature change on lipid droplets in palmitate-treated adipocytes. In the palmitate-induced adiposity model, although excessive ROS and lipid peroxidation has been attenuated by temperature decrease (P<0.01), it still does not positively modulate lipid droplet size (P>0.05) and remedy the palmitate damage induced cell death (P<0.01). These findings provide preliminary support for potential interventions based on temperature manipulation for cell metabolism of adipocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Thermal Biology - Volume 58, May 2016, Pages 1–7
نویسندگان
, , , , , , ,