کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2847404 | 1167360 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: l-carbocisteine inhibits respiratory syncytial virus infection in human tracheal epithelial cells l-carbocisteine inhibits respiratory syncytial virus infection in human tracheal epithelial cells](/preview/png/2847404.png)
To examine the effects of l-carbocisteine on airway infection with respiratory syncytial (RS) virus, human tracheal epithelial cells were pretreated with l-carbocisteine and infected with RS virus. Viral titer, virus RNA, and pro-inflammatory cytokine secretion, including interleukin (IL)-1 and IL-6, increased with time after infection. l-carbocisteine reduced the viral titer in the supernatant fluids, the amount of RS virus RNA, RS virus infection susceptibility, and the concentration of pro-inflammatory cytokines induced by virus infection. l-carbocisteine reduced the expression of intercellular adhesion molecule (ICAM)-1, an RS virus receptor, on the cells. However, l-carbocisteine had no effects on the expression of heparan sulfate, a glycosaminoglycan that binds to the RS virus attachment protein, or on the amount of intracellular activated-RhoA, isoform A of the Ras-homologous family, that binds to the RS virus fusion protein. These findings suggest that l-carbocisteine may inhibit RS virus infection by reducing the expression of ICAM-1. It may also modulate airway inflammation during RS virus infection.
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► A mucolytic agent, l-carbocisteine, inhibited RS virus infection and decreased the susceptibility of cultured human tracheal epithelial cells to RS virus infection by reducing levels of ICAM-1, a RS virus receptor.
► l-carbocisteine also reduced the baseline and RS virus infection-induced secretion of pro-inflammatory cytokines, including IL-1β, IL-6 and IL-8.
► l-carbocisteine may modulate airway inflammation after RS virus infection.
Journal: Respiratory Physiology & Neurobiology - Volume 180, Issue 1, 15 January 2012, Pages 112–118