کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2926928 1575810 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice
ترجمه فارسی عنوان
مدل تجربی انتقال از هیپرتروفی قلب جبران شده به شکست ناشی از تنگی عرضی آئورت در موش
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی

BackgroundTransverse aortic constriction (TAC) operation is used as an experimental model of left ventricular (LV) hypertrophy and LV failure in mice. The severity of LV remodeling or failure may depend on the degree of TAC, but is variable among operated animals. Therefore, we tried to identify the optimal diameter of TAC to create this model with ease and high reproducibility.Methods and resultsTo produce TAC in C57BL/6J mice (7–9 weeks, body weight 19–26 g, n = 109), a 7–0 nylon suture ligature was tightly tied around the transverse aorta against needles with 3 different diameters (mm); 0.40, 0.385 and 0.375. LV wall thickness, end-diastolic dimension, fractional shortening were measured by echocardiography. At 4 weeks after TAC, no mouse with the 0.400 mm gauge progressed in LV failure. The 0.385 mm pin gauge mouse kept a more survival rate compared with the 0.375 mm (59% vs 48%), representing same efficient in LV failure. With the 0.385 mm pin gauge, hearts of mice remained LV hypertrophy at 1 week after TAC, followed by LV failure at 4 weeks.ConclusionTAC with the diameter of 0.385 mm can effectively induce the transition from LV hypertrophy to failure in mice with relatively preserved survival.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: IJC Heart & Vasculature - Volume 11, June 2016, Pages 24–28
نویسندگان
, , , , , , , , , , , , ,