کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2929538 | 1576205 | 2013 | 10 صفحه PDF | دانلود رایگان |
BackgroundsAlthough the rescue of cellular senescence during ex vivo expansion of human-derived cardiac progenitor cells (hCPC) is critical for the application of autologous stem cell therapy in cardiovascular disease, the underlying molecular pathways during replicative senescence in hCPC have not been fully defined. Thus, we examined whether the regulation of mitogen-activated protein kinases activation could facilitate the recovery of human c-kit-positive hCPCs (hCPCc-kit +) and whether senescence is reactive oxygen species (ROS)-dependent or -independent.Methods and resultsTo investigate the molecular pathways of replicative cellular senescence, we first evaluated cellular senescence in ex vivo-expanded hCPCc-kit + by using senescence-associated β-galactosidase (SA-β-gal) activity with enlarged cytoplasm and observed increased expression of cell senescence-related pivotal molecules, including TP53, cleavage Mdm2 (cMdm2), and Mdm2. Unexpectedly, we found that the extracellular signal-regulated kinase (ERK) was markedly activated in aged hCPCc-kit +, with reduced proliferative activity. SA-β-gal activity and cytoplasm size in senescent hCPCc-kit + were significantly reduced, with reduced TP53 and cMdm2 expression after treatment with a specific ERK inhibitor (U0126). We examined whether the signaling in ERK inhibitory rescue of hCPCc-kit + senescence is ROS-dependent. Interestingly, the increased ROS level was not changed after treatment with a specific ERK inhibitor. Similarly, the increased expression levels of endogenous antioxidant enzymes, e.g., peroxiredoxin (Prdx)-1 and 2, in senescent hCPCc-kit + were not changed after treatment with a specific ERK inhibitor.ConclusionsFrom the above results, we conclude that the specific inhibition of ERK during cellular senescence might rescue bioactivities of senescent hCPCc-kit + in a ROS-independent manner.
Journal: International Journal of Cardiology - Volume 169, Issue 1, 25 October 2013, Pages 73–82