The subepicardial microcirculation in heterotopically transplanted mouse hearts: An intravital multifluorescence microscopy study

We introduce a novel approach to visualize in detail the murine coronary microcirculation in vivo by multifluorescence microscopy. Our data demonstrate that prolonged cold ischemia provokes posttransplant capillary no-reflow, leukocytic inflammation, and persistent tissue hypoxia.