کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2993204 1179891 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence for markers of hypoxia and apoptosis in explanted human carotid atherosclerotic plaques
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Evidence for markers of hypoxia and apoptosis in explanted human carotid atherosclerotic plaques
چکیده انگلیسی

ObjectiveApoptosis and inflammation are important features of atherosclerotic plaques. We investigated whether a common signal molecule can trigger these two apparently separate pathways. Hypoxia inducible factor (HIF-1α) is known to participate in atherosclerosis and to stimulate apoptosis signal-regulating kinase 1 (ASK-1), one of the mitogen-activated protein kinases, which is activated by various extracellular stimuli and involved in a variety of cellular function.MethodsWe tested carotid artery specimens from 50 subjects who underwent angioplasty and five age-matched controls for either Western blot or histologic analysis. The hypoxic status was investigated by means of HIF-1α expression in carotid specimens.ResultsHIF-1α was significantly upregulated in carotid specimens with respect to controls (P < .05), ASK-1 was detected in plaques of any composition from lipidic to calcific, and this expression increased with the stage of the plaque and with the expression of inflammatory (p-ERK, RANK-L, OPG) and apoptotic molecules (caspase 9, p-p-38, and p-JNK).ConclusionOur data suggest that hypoxia is the key regulating factor that triggers inflammation as well as apoptosis in the human atherosclerotic plaque.

Clinical RelevanceThis study adds evidence that HIF-1α is important in the oxidative stress response in the developing atherosclerotic plaque as it modulates both inflammatory and mitochondrion-related apoptotic pathways. Future therapy targeting HIF-1α may limit atherosclerosis development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Vascular Surgery - Volume 52, Issue 4, October 2010, Pages 1015–1021
نویسندگان
, , , , , , , ,