کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3009787 1181497 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypothermia improves ventricular myocyte contractility under conditions of normal perfusion and after an interval of ischemia
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Hypothermia improves ventricular myocyte contractility under conditions of normal perfusion and after an interval of ischemia
چکیده انگلیسی

AimRecent investigations have reported improved myocardial function during hypothermia following resuscitation from cardiac arrest. The effects of hypothermia on myocyte contractility were investigated under conditions of normal perfusion and after a 10 min interval of ischemia.MethodsVentricular myocytes were obtained from 10 male Sprague–Dawley rats weighing 400 ± 50 g. The myocytes were randomized to be perfused at: 37 °C, 34 °C, 32 °C, or 30 °C. A subsequent set of myocytes was subjected to 10 min of ischemia at 37 °C, prior to being randomized to reperfusion at: 37 °C, 34 °C, 32 °C or 30 °C. Myocyte contractility was expressed as length-shortening percentage. Intracellular Ca2+ transients were assessed in a separate group of myocytes preloaded with Fura-2/AM. Sensitivity to Ca2+ was tested by increasing perfusate Ca2+ content, i.e. 0.5 mM, 1 mM and 2 mM.ResultsDuring normal perfusion and following reperfusion after 10 min of ischemia, myocyte contractility increased at 34 °C compared to 37 °C (P < 0.01). When the perfusion temperature was decreased to 32 °C and 30 °C, contractility further increased (P < 0.001). Intracellular Ca2+ transients were greater during perfusion at 34 °C compared to those at 37 °C (P < 0.001) and further increased at 30 °C (P < 0.001). Increases in extracellular Ca2+ concentration from 0.5 mM to 2 mM resulted in greater myocyte contractility during perfusion at 30 °C compared to that observed at 37 °C (P < 0.001). Effects of hypothermia on intracellular Ca2+ transients and sensitivity to Ca2+ persisted after ischemia.ConclusionsHypothermia improved myocyte contractility, intracellular Ca2+ transients and sensitivity to Ca2+ under conditions of normal perfusion and following reperfusion after 10 min of ischemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Resuscitation - Volume 81, Issue 7, July 2010, Pages 898–903
نویسندگان
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