Synthesis of CuO nanoparticles and fabrication of nanostructural layer biosensors for detecting Aspergillus niger fungi

Copper oxide (CuO) nanoparticles and nanolayers were synthesized by sol–gel and spray pyrolysis methods, respectively. The structure and morphology of the prepared samples were characterized using XRD, SEM and TEM analysis. Aspergillus niger fungi were grown in an appropriate medium and exposed to the synthesized samples in a closed glass vessel. The boisoning properties of the nano systems were investigated by measuring their electrical resistance at regular time intervals and different temperatures. Further studies were made on the effects of CO2 and humidity on the sensing properties, using CaCo3 and Silica gel. The considerable changes observed in the electrical resistance of the prepared samples, in the presence of Aspergillus niger fungi, support our proposed system as a biosensor.