Can Mineral Trioxide Aggregate and Nanoparticulate EndoSequence Root Repair Material Produce Injurious Effects to Rat Subcutaneous Tissues?

MTA and ERRM root repair materials cause injurious effect to rat subcutaneous tissues.
• MTA and ERRM cause moderate to severe injurious effect to tissues at 7 days after their implantation.
• The inflammatory reaction decreased after 30 days.
• MTA was more injurious to subcutaneous tissues than ERRM.

IntroductionThe aim of this study was to evaluate the injurious effects of mineral trioxide Aggregate (MTA) and EndoSequence Bioceramic Root Repair Material (ERRM; Brassler USA, Savannah, GA) 7 and 30 days after their implantation into rat subcutaneous tissues.MethodsTwelve Wistar rats were selected for the present study. Each animal received 3 implants: one contained MTA, one contained ERRM, and one was an empty tube that served as a control. Half of the animals were killed after 7 days, and the remaining animals were killed 30 days after implantation. Histologic sections prepared from the skin specimens were stained with H&E, toluidine blue, Masson trichrome, and Congo red. The data were statistically analyzed with 1-way analysis of variance and paired t tests. The P value for significance was set at .05.ResultsAfter 7 days, MTA produced a significantly greater inflammatory reaction that involved the deposition of amyloidlike protein and an increase in the mast cell population compared with ERRM (P < .05). After 30 days, the ERRM group exhibited significantly reduced inflammatory reactions compared to the MTA groups (P < .05). Areas of mononuclear cell aggregation, abscess formation, and necrosis were observed more frequently in the MTA group. The thickness of the fibrous capsule was significantly increased in the MTA compared with the ERRM groups (P < .05). Amyloidlike proteins were more frequently observed around the fibrous capsule and subdermal blood vessels and were more frequently deposited in the MTA than the ERRM specimens.ConclusionsThe findings of the present study suggest that both ERRM and MTA cause an injurious effect when implanted in rat subcutaneous tissues after 7 and 30 days. ERRM is significantly less injurious to tissues than MTA.