کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3147647 | 1197372 | 2016 | 8 صفحه PDF | دانلود رایگان |

• A model of periapical periodontitis in rats was successfully established by sealing Enterococcus faecalis into the pulp chamber for the first time, and the NLRP3 inflammasome plays an important role in the progression of inflammatory process.
• Without the presence of adenosine triphosphate, E. faecalis lipoteichoic acid (LTA) could activate the NLRP3 inflammasome in phagocytes directly.
• The molecular mechanism of the LTA-regulated NLRP3 inflammasome was because it first activates the nuclear factor kappa B signaling pathway, and the findings may provide some clues to treat the disease.
IntroductionWe wished to examine the effects of the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome on periapical periodontitis induced by Enterococcus faecalis and to investigate the molecular mechanisms of lipoteichoic acid (LTA) derived from E. faecalis on the expression of the NLRP3 inflammasome.MethodsA model of periapical periodontitis by sealing E. faecalis into the pulp chambers of rats was established. We then examined the relationship between the expression, location, distribution, and concentration of NLRP3, caspase-1, and interleukin 1β with the inflammatory progression by immunohistochemistry and undertook correlation analyses. RAW264.7 cells were cultured in the absence or presence of LTA together with or without nuclear factor kappa B (NF-κB) inhibitor BAY 11-7082; NLRP3 inflammasome expression was measured by Western blotting, the enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction. An immunofluorescence study was conducted to further detect whether NF-κB can be completely inhibited by BAY 11-7082 or activated by LTA.ResultsAn animal model of periapical periodontitis was established successfully. Expression of NLRP3, caspase-1, and interleukin 1β protein was observed in the inflamed area. The expression of these 3 proteins had a significant positive correlation (P < .05). Overall, our results showed that, compared with the negative control group, LTA could directly activate expression of messenger RNA and protein of the NLRP3 inflammasome (P < .05), whereas BAY 11-7082 inhibited it (P < .05).ConclusionsOur results suggested that LTA can act as a directly stimulating factor associated with expression of the NLRP3 inflammasome during periapical periodontitis, which is mainly linked with the NF-κB signaling activation pathway.
Journal: Journal of Endodontics - Volume 42, Issue 7, July 2016, Pages 1093–1100