کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3149012 1197430 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biofilm Formation of Oral and Endodontic Enterococcus faecalis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Biofilm Formation of Oral and Endodontic Enterococcus faecalis
چکیده انگلیسی

Biofilms are complex aggregations of microorganisms attached to a surface. The formation of biofilms might facilitate certain survival and virulence characteristics under some situations. This study tested the hypothesis that the ability of Enterococcus faecalis to form biofilms is related to the source of the strains. E. faecalis strains recovered from root canals (n = 33), the oral cavity (n = 21), and non-oral/non-endodontic sources (n = 16) were studied. Biofilms were grown in tryptic soy broth in 96-well plates for 24 hours at 37°C, fixed with Bouin’s fixative, and stained with 1% crystal violet. Optical density at 570 nm (OD570) was measured by using a microtiter plate reader. Experiments were performed in quadruplicate on three occasions and mean OD570 readings determined for each strain. There were no statistically significant differences between groups (p = 0.066, Kruskal-Wallis). Within the root canal and oral isolates there were no significant associations between biofilm formation and the presence of the virulence determinants asa, cylA, esp, and gelE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Endodontics - Volume 33, Issue 7, July 2007, Pages 815–818
نویسندگان
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