کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3149268 1197446 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of Mineral Trioxide Aggregate on Mesenchymal Stem Cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Effect of Mineral Trioxide Aggregate on Mesenchymal Stem Cells
چکیده انگلیسی

IntroductionMineral trioxide aggregate (MTA) is known to stimulate the hard tissue repair process. The purpose of this study was to evaluate the ability of MTA to support the adhesion, proliferation, and migration of human bone marrow–derived mesenchymal stem cells (hMSCs).MethodsWhite ProRoot MTA and white Portland cement were mixed and left to set 24 hours. MSCs were cultured on the samples and observed after 24 hours by confocal laser scanning microscopy (CLSM) by using the cytoskeleton marker CellTracker. Cell proliferation was evaluated by means of alamar blue assay in the presence and absence of differentiation medium during a period of 28 days, and cells seeded on polystyrene culture wells were the control. To assess the effect on migratory ability of hMSCs, a transwell migration assay was performed for 18 hours, positioning MTA and Portland cement in 6-well plates and the cells in 8-μm pore inserts.ResultshMSCs observed under CLSM showed attachment and spread activity on the upper surface of the MTA. Cell proliferation was significantly higher on MTA than on Portland cement. A rate proliferation increase of the MTA group compared with the control was observed after 14 days in presence of basic medium, whereas the same effect was reached after 21 days in presence of differentiation medium. Moreover, MTA was able to enhance cell migration significantly more than Portland cement.ConclusionsOur findings suggest that MTA was able to assist hMSC adhesion, growth, and migration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Endodontics - Volume 36, Issue 11, November 2010, Pages 1839–1843
نویسندگان
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