کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3212454 | 1407115 | 2016 | 10 صفحه PDF | دانلود رایگان |
• It is not well defined whether dermis-derived factors regulate the proliferation and differentiation of human melanocytes.
• PDGF-BB stimulated the proliferation of cultured melanoblasts, but inhibited the proliferation of melanocytes.
• PDGF-BB promoted melanocyte differentiation through the stimulation of the expressions of tyrosinase and tyrosinase-related protein-1.
• PDGF-BB regulates the proliferation and differentiation of human melanocytes in a differentiation-stage-specific manner.
BackgroundAlthough many kinds of keratinocyte-derived factors are known to regulate the proliferation and differentiation of human melanocytes, it is not well defined whether dermis-derived factors work in a similar way.ObjectiveThe aim of this study is to clarify whether dermal factors are involved in regulating the proliferation and differentiation of human melanocytes.MethodsHuman epidermal melanoblasts were cultured serially in a serum-free growth medium. Platelet-derived growth factor-BB (PDGF-BB) was supplemented to the medium, and the effects on the proliferation of melanoblasts/melanocytes and the differentiation of melanocytes were studied.ResultsPDGF-BB stimulated the proliferation of melanoblasts cultured in melanoblast-proliferation medium, but inhibited the proliferation of melanocytes cultured in melanocyte-proliferation medium. By contrast, PDGF-BB stimulated the differentiation, dendritogenesis, and melanogenesis of melanocytes through the stimulation of tyrosinase activity and the expressions of tyrosinase and tyrosinase-related protein-1.ConclusionThese results suggest that PDGF-BB regulates the proliferation and differentiation of human melanocytes in a differentiation-stage-specific manner. PDGF-BB seems to be one of the dermal factors that regulate the proliferation and differentiation of human melanocytes.
Journal: Journal of Dermatological Science - Volume 83, Issue 3, September 2016, Pages 200–209