کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3212656 1203190 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative proteogenomic profiling of epidermal barrier formation in vitro
ترجمه فارسی عنوان
مشخصات پروتوژنومیک کمی از تشکیل مانع اپیدرمی در شرایط آزمایشگاهی
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی امراض پوستی
چکیده انگلیسی


• Proteomics and transcriptomics were performed for a model of epidermal development.
• 3661 proteins were identified across three developmentally crucial time points.
• Quantitation revealed over 1200 differentially abundant proteins.
• 574 proteins matched transcript abundance on a rank-order basis.

BackgroundThe barrier function of the epidermis is integral to personal well-being, and defects in the skin barrier are associated with several widespread diseases. Currently there is a limited understanding of system-level proteomic changes during epidermal stratification and barrier establishment.ObjectiveHere we report the quantitative proteogenomic profile of an in vitro reconstituted epidermis at three time points of development in order to characterize protein changes during stratification.MethodsThe proteome was measured using data-dependent “shotgun” mass spectrometry and quantified with statistically validated label-free proteomic methods for 20 replicates at each of three time points during the course of epidermal development.ResultsOver 3600 proteins were identified in the reconstituted epidermis, with more than 1200 of these changing in abundance over the time course. We also collected and discuss matched transcriptomic data for the three time points, allowing alignment of this new dataset with previously published characterization of the reconstituted epidermis system.ConclusionThese results represent the most comprehensive epidermal-specific proteome to date, and therefore reveal several aspects of barrier formation and skin composition. The limited correlation between transcript and protein abundance underscores the importance of proteomic analysis in developing a full understanding of epidermal maturation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dermatological Science - Volume 78, Issue 3, June 2015, Pages 173–180
نویسندگان
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