کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3213126 1203219 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extracellular epimorphin modulates epidermal differentiation signals mediated by epidermal growth factor receptor
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی امراض پوستی
پیش نمایش صفحه اول مقاله
Extracellular epimorphin modulates epidermal differentiation signals mediated by epidermal growth factor receptor
چکیده انگلیسی

BackgroundThe epidermal stratification/differentiation program is initiated in keratinocytes by a basement membrane-detachment cue and subsequently controlled by spatially and temporally regulated signaling molecules. The vital signals for the developmental behavior of the epidermis include those mediated by epidermal growth factor receptor (EGFR); however, regulatory elements responsible for activation have not yet been fully elucidated.ObjectiveThe objectives of this study were (1) to assess the effects of EGFR activation on epidermal differentiation, (2) to study the effects of epimorphin on the level of EGFR signaling degree dependent on matrix engagement, and (3) to address the impact of epimorphin modulation on EGFR-driven epidermal differentiation in a three-dimensional (3D) organotypic skin model.MethodsWe constructed skin-equivalent models with a well-stratified differentiated epidermis and utilized them to evaluate the epidermal behaviors.ResultsExtracellularly secreted epimorphin was identified as a strong candidate for signaling pathway involvement. In a 3D epidermis model, EGF stimulation was sufficient for epidermal stratification. However, overactivation of EGFR led to irregular multicellular arrangements with an abnormal differentiation profile, which appeared to be reorganized back to the normal epidermal phenotype by extracellular epimorphin. Extracellular epimorphin interestingly attenuated EGF-stimulated EGFR phosphorylation, cell growth, and migration in adherent cells. In contrast to the results of adhesion culture, extracellular epimorphin reinforced EGFR activation in suspended cells.ConclusionsThese results demonstrate that epimorphin modulates the signaling pathways mediated by EGFR for epidermal tissue organization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dermatological Science - Volume 69, Issue 3, March 2013, Pages 236–242
نویسندگان
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