کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3262311 1207729 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bile canalicular abnormalities in the early phase of a mouse model of sclerosing cholangitis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی غدد درون ریز، دیابت و متابولیسم
پیش نمایش صفحه اول مقاله
Bile canalicular abnormalities in the early phase of a mouse model of sclerosing cholangitis
چکیده انگلیسی

BackgroundThe bile canaliculus is the smallest and first biliary channel and is formed by two or three adjacent hepatocytes. Previous studies of chronic cholangiopathies such as primary sclerosing cholangitis have focused on the bile ductules. However, little is known about the pathological alterations in bile canaliculi in the early phase of cholangiopathies.AimTo characterize the bile canalicular morphology in the early phase of sclerosing cholangitis we used 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced mouse model of sclerosing cholangitis.MethodsMice were fed a diet with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (0.1%). Serum biochemical, histological, immunohistochemical, and electron microscopic analyses were performed 1, 2, 4, and 7 days after feeding.ResultsAll experimental groups showed significantly increased serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase levels. From day 1, bile canalicular abnormalities such as dilatation and meandering and loss of microvilli were observed. After bile canalicular abnormalities had appeared, substantial infiltration of inflammatory cells was observed amongst the necrotic cells and periductal region. After these inflammatory changes, cholangiocytes proliferated in the portal area and formed ductular reactions. Finally, periductal fibrosis appeared.ConclusionThis study provides novel evidence of the occurrence of bile canalicular abnormalities during the early phase of sclerosing cholangitis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Digestive and Liver Disease - Volume 45, Issue 3, March 2013, Pages 216–225
نویسندگان
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