کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3297260 1209882 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nitric Oxide–Sensitive Guanylyl Cyclase Is Dispensable for Nitrergic Signaling and Gut Motility in Mouse Intestinal Smooth Muscle
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های گوارشی
پیش نمایش صفحه اول مقاله
Nitric Oxide–Sensitive Guanylyl Cyclase Is Dispensable for Nitrergic Signaling and Gut Motility in Mouse Intestinal Smooth Muscle
چکیده انگلیسی

Background & AimsThe nitric oxide–guanosine 3',5'-cyclic monophosphate (cGMP) signaling pathway has an important role in the control of smooth muscle tone. NO is produced by NO synthases and acts as a major inhibitory neurotransmitter in the gastrointestinal (GI) tract. The main target, NO-sensitive guanylyl cyclase (NO-GC), is stimulated by NO to produce the intracellular messenger cGMP. We investigated the role of NO-GC in nitrergic relaxation and GI motility.MethodsWe tested relaxation of GI smooth muscle in mice that do not express NO-GC or mice with disruption of NO-GC specifically in smooth muscle cells. Different segments of the GI tract (fundus, lower esophageal sphincter, pyloric sphincter, and duodenum) were used in isometric force studies. NO donors and electrical field stimulation were used to assess nitrergic signaling. Whole-gut transit time was measured as an indicator of GI motility.ResultsMice that lack NO-GC do not have NO-induced relaxation of GI smooth muscle. Gut transit time was increased, resulting in GI dysfunction. Surprisingly, in mice that lack NO-GC specifically in smooth muscle, NO-induced relaxation was reduced only slightly, and whole-gut transit time was unchanged compared with wild-type mice.ConclusionsLack of NO-GC in smooth muscle cells does not impair NO-induced relaxation of GI tissues or GI motility. The NO receptor guanylyl cyclase in GI smooth muscle is therefore dispensable for nitrergic signaling in mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 140, Issue 5, May 2011, Pages 1608–1617
نویسندگان
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