کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3299057 | 1209921 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for the Role of Interferon-alfa Production by Dendritic Cells in the Th1 Response in Celiac Disease
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کلمات کلیدی
RT-PCRTLR9TGFLPMCToll-like receptor-9TNFinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینtransforming growth factor - تبدیل فاکتور رشدlamina propria mononuclear cell - سلول تک هسته ای لامین پروپریاDendritic cell - سلول دندریتیکtumor necrosis factor - فاکتور نکروز تومورreverse-transcription polymerase chain reaction - واکنش زنجیره پلیمراز معکوس رونویسی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Background & Aims: Dendritic cells (DCs) play a crucial role in immune responses by controlling the extent and type of T-cell response to antigen. Celiac disease is a condition in which T-cell immunity to gluten plays an important pathogenic role, yet information on DCs is scant. We examined mucosal DCs in celiac disease in terms of phenotype, activation/maturation state, cytokine production, and function. Methods: Mucosal DCs from 48 celiacs and 30 controls were investigated by flow cytometry. In situ distribution of DCs was analyzed by confocal microscopy. Interferon (IFN)-alfa, interleukin (IL)-4, IL-5, IL-12p35, IL-12p40, IL-18, IL-23p19, IL-27, and transforming growth factor-β transcripts were measured by real-time reverse-transcription polymerase chain reaction in sorted DCs. DC expression of IL-6, IL-12p40, and IL-10 was assessed by intracellular cytokine staining. The effect of IFN-alfa and IL-18 blockade on the gluten-induced IFN-γ response in celiac biopsy specimens grown ex vivo also was investigated. Results: Mucosal DCs were increased in untreated, but not treated, celiacs. The majority of them were plasmacytoid with higher levels of maturation (CD83) and activation (CD80/CD86) markers. Higher transcripts of Th1 relevant cytokines, such as IFN-alfa, IL-18, and IL-23p19, were produced by celiac DCs, but because IL-12p40 was undetectable, a role for IL-23 is unlikely. Intracellular cytokine staining of celiac DCs showed higher IL-6, but lower IL-10 expression, and confirmed the lack of IL-12p40. Blocking IFN-alfa inhibited IFN-γ transcripts in ex vivo organ culture of celiac biopsy specimens challenged with gluten. Conclusions: These data suggest that IFN-alfa-producing DCs contribute to the Th1 response in celiac disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 133, Issue 4, October 2007, Pages 1175-1187
Journal: Gastroenterology - Volume 133, Issue 4, October 2007, Pages 1175-1187
نویسندگان
Antonio Di Sabatino, Karen M. Pickard, John N. Gordon, Virginia Salvati, Giuseppe Mazzarella, Robert M. Beattie, Anna Vossenkaemper, Laura Rovedatti, Nicholas A.B. Leakey, Nicholas M. Croft, Riccardo Troncone, Gino R. Corazza, Andrew J. Stagg,