کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3315562 1211273 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
NADPH oxidase is not an essential mediator of oxidative stress or liver injury in murine MCD diet-induced steatohepatitis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های گوارشی
پیش نمایش صفحه اول مقاله
NADPH oxidase is not an essential mediator of oxidative stress or liver injury in murine MCD diet-induced steatohepatitis
چکیده انگلیسی

Background/AimsHepatic oxidative stress is a key feature of metabolic forms of steatohepatitis, but the sources of pro-oxidants are unclear. The NADPH oxidase complex is critical for ROS generation in inflammatory cells; loss of any one component (e.g., gp91phox) renders NADPH oxidase inactive. We tested whether activated inflammatory cells contribute to oxidant stress in steatohepatitis.Methodsgp91phox−/− and wildtype (wt) mice were fed a methionine and choline-deficient (MCD) diet. Serum ALT, hepatic triglycerides, histopathology, lipid peroxidation, activation of NF-κB, expression of NF-κB-regulated genes and macrophage chemokines were measured.ResultsAfter 10 days of MCD dietary feeding, gp91phox−/− and wt mice displayed equivalent hepatocellular injury. After 8 weeks, there were fewer activated macrophages in livers of gp91phox−/− mice than controls, despite similar mRNA levels for MCP and MIP chemokines, but fibrosis was similar. NF-κB activation and increased expression of ICAM-1, TNF-α and COX-2 mRNA were evident in both genotypes, but in gp91phox−/− mice, expression of these genes was confined to hepatocytes.ConclusionsA functional NADPH oxidase complex does not contribute importantly to oxidative stress in this model and therefore is not obligatory for induction or perpetuation of dietary steatohepatitis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hepatology - Volume 46, Issue 2, February 2007, Pages 304–313
نویسندگان
, , , ,