کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3405856 | 1223486 | 2015 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evaluation of viral load in saliva from patients with chronic hepatitis C infection Evaluation of viral load in saliva from patients with chronic hepatitis C infection](/preview/png/3405856.png)
SummaryHepatitis C virus can be detected in blood and other bodily fluids, such as saliva. The aim of this study was to detect and quantify the HCV-RNA in saliva and plasma from patients with chronic hepatitis C infections, as well as check the level of viral load in sex groups (age, ethnicity and virus subtypes). Whole saliva and blood from 70 patients with chronic hepatitis C infections attended at the department of gastroenterology from University Hospital. The HCV-RNA load was performed by qRT-PCR using Sybr Green I master mix. HCV-RNA was detected in 80% (56/70) of patients in saliva and 92.85% (65/70) in plasma. The median of the viral load in the plasma was of 4.87 log10, and in saliva, it was 3.32 log10, (p = 0.0005). Female patients and black patients exhibited a negative correlation between the HCV-RNA load in saliva vs. the HCV-RNA load in plasma (r = −0.3172, CI95% −0.6240 to −0.03736, p = 0.0491) and (r = −0.3141; IC95% −0.6069 to −0.05926; p = 0.0209), respectively. HCV-RNA was detected and quantified in saliva samples, and according to the quantification levels, saliva may be a possible transmission source of HCV, particularly in women and people of black ethnicity who develop chronic HCV infections.
Journal: Journal of Infection and Public Health - Volume 8, Issue 5, September–October 2015, Pages 474–480