کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3441053 1595030 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An autoregulatory element maintains HOXA10 expression in endometrial epithelial cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
An autoregulatory element maintains HOXA10 expression in endometrial epithelial cells
چکیده انگلیسی

ObjectiveHOXA10 is necessary for endometrial receptivity and regulated by sex steroids. Secretory phase HOXA10 expression increases in endometrial epithelial cells, despite the loss of progesterone receptor. Stromal-epithelial molecular communication likely transmits progesterone signaling from progesterone receptor containing stromal cells to epithelium. Here we investigated an alternative hypothesis, persistent HOXA10 expression by autoregulation.Study designNested segments of the HOXA10 5′ regulatory region were cloned into a pGL3-Luciferase reporter construct and tested for HOXA10-induced reporter activity. Direct binding was assayed by electrophoretic mobility shift assay.ResultsOne 370 base pair element drove reporter gene expression specifically in response to HOXA10 in Ishikawa cells but not in BT-20 cells. This element contained a site that bound HOXA10 protein.ConclusionHOXA10 expression is driven by an autoregulatory element in the 5′ regulatory region of the gene. Autoregulation is a novel alternative molecular mechanism by which steroid-induced gene expression can be maintained in the absence of steroid receptors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: American Journal of Obstetrics and Gynecology - Volume 194, Issue 4, April 2006, Pages 1100–1107
نویسندگان
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