کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3447599 1595531 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of Subchronic Exposure to Static Magnetic Field on Testicular Function in Rats
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Effects of Subchronic Exposure to Static Magnetic Field on Testicular Function in Rats
چکیده انگلیسی

BackgroundThe aim of this study was to investigate the effect of static magnetic field (SMF) exposure on testicular function, antioxidant status and DNA oxidation in rats.MethodsMale adult rats were exposed to SMF (128 mT; 1 h/day for 30 days). After sacrifice, the epididymal sperm number was counted. Testosterone concentration in plasma and testis was measured by radioimmunoassay. MDA level and GPx, CAT and SOD activities were used as markers of oxidative stress in testis. The 8-oxo-dG level is measured by the HPLC-EC system.ResultsSubchronic exposure to SMF has no effect on epididymal sperm count, spermatozoa motility and genital organ weight. In contrast, SMF induces a decrease of testicular and plasmatic testosterone levels, respectively (1.48 ± 0.56 vs. 4.66 ± 0.51 ng/g, p<0.05; 0.97 ± 0.16 vs. 1.64 ± 0.18 ng/mL, p<0.05). Exposed rats displayed an increase of malondialdehyde (2.01 ± 0.03 vs. 1.47 ± 0.06 μmol/g protein, p<0.05), metallothioneins (1.04 ± 0.22 vs. 0.37 ± 0.06 μg/g, p<0.05) and 8-oxo-dG concentrations (3.38 ± 0.30 vs. 2.36 ± 0.28 8-oxo-dG/106 bases, p<0.05) in the testis. In the gonad, SMF decreases the CAT (14.33 ± 1.16 vs. 21.67 ± 2.05 U/mg protein, p<0.05), GPx (177.40 ± 5.97 vs. 237.20 ± 15.65 U/mg protein, p<0.05) and mitochondrial Mn–SOD (2.95 ± 0.10 vs. 3.53 ± 0.29 U/mg protein, p<0.05) activities. However, cytosolic CuZn–SOD activity is unaffected.ConclusionsSubchronic exposure to SMF failed to alter spermatogenesis in rat testis. In contrast, the same treatment decreased testosterone levels and induced DNA oxidation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Medical Research - Volume 37, Issue 8, November 2006, Pages 947–952
نویسندگان
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