Effect of different thawing procedures on the quality and fertility of the bull spermatozoa

ObjectiveTo improve the indicators of motility, survival and fertilizing ability of spermatozoa by optimizing temperature factors and the duration of exposure at unfreezing straws.MethodsStraws by volume 0.25 mL were thawed at water bath temperatures at 65 °C, 67 °C and 70 °C for 6–7 seconds and at 75 °C for 4–6 seconds. Impact of exposure time and temperature thawing in the water bath on motility and survival of spermatozoa were studied.ResultsStudies indicate that for the procedure of defrost water bath straws in seven seconds for temperature conditions of 65 °C, 67 °C and 70 °C, indicators of progressive motility and absolute survival rate were significantly higher than for the control group an average on 11.4 % (P<0.01). Optimum exposure time (6–7 seconds) and temperature range (65–70 °C) defrosting semen doses were defined.ConclusionsOwing obtained the positive result, method of thawing was developed which increases the indicators of motility, survival and fertilizing capacity of bull semen.