Effect of sera of normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) on in vitro maturation of buffalo, sheep and goat oocytes

ObjectiveTo examine the oocytes maturation capacity of buffalo, sheep and goat using media containing sera of three different groups of buffaloes (regularly cycling, pregnant and repeat breeding).MethodsThe buffalo, sheep and goat oocytes were matured under suitable conditions in medium containing sera of regularly cycling, pregnant and repeat breeding buffaloes.ResultsThe oocytes maturation rate containing buffalo oocytes cultured in media containing sera of the control group and regularly cycling group were not significantly different. However when oocytes cultured in the media containing sera of pregnant buffaloes the maturation rate were significantly declined. Further significant declined in maturation rate were observed when oocytes cultured in media containing sera of repeat breeding buffaloes. When sheep and goat oocytes cultured in the media containing control, pregnant and regularly cycling animals sera the oocytes maturation rate were not significantly different. A significant decline in maturation rate of sheep and goat oocytes were observed, when oocytes cultured in media containing sera of repeat breeding buffaloes.ConclusionWe may conclude that serum collected from normal cycling buffaloes can be used for oocytes maturation in both homogeneous and heterogeneous species.