Possible effect of tea plant parasite, Scurrula atropurpurea (Blume) Danser, on growth inhibition of culture HeLa cells in vitro through DNA repair and apoptosis intrinsic pathways mechanism

ObjectiveTo study the possible in vitro effect of tea plant parasite [Scurrula atropurpurea (Blume) Danser] extract on HeLa cells culture growth inhibition through DNA repair and apoptosis intrinsic pathways mechanism.MethodsFlow cytometry assay were performed for those analyses.ResultsFor DNA repair pathway mechanism, treatment of tea plant parasite extract decreased MDM2 expression and increased p21 expression. And for apoptosis markers measurement on bax and bcl-2 showed an opposite result, increasing on bax and decreasing on bcl-2 expression. Almost all doses treatment in all measurement showed significantly result.ConclusionsThis result showed an effect of tea plant parasite extract on inhibition of cells HeLa culture growth through DNA repair and apoptosis intrinsic pathways mechanism.