کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3454446 1595966 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histopathology of mammary gland in Staphylococcus aureus induced mastitis in mice
ترجمه فارسی عنوان
هیستوپاتولوژی غدد پستان در موشهای مبتلا به استافیلوکوکوس اورئوس موست
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی

ObjectiveTo investigate the histopathological changes of mouse mammary tissue damage in dose and durational pathogenicity of Staphylococcus aureus (S. aureus) inoculums.MethodsThe minimal tissue inflammation dose of S. aureus inoculum was determined by inoculating the lactating mice mammary gland to observe the mortality rate at 24, 48, 72 and 96 h of infection for each inoculum dilutions. To study dose pathogenicity, lactating mice were divided into one control and four induced groups of 5 mice each. S. aureus inoculums doses of 0.8×101, 1.4×102, 2×102 and 2.8×103 colony forming unit were inoculated to the induced groups II, III, IV and V respectively. Mice were sacrificed and mammary gland tissues were harvested after 48 h of infection. Similarly to study of durational pathogenicity, a dose of 2×102 colony forming unit of S. aureus inoculums was inoculated to the induced groups II, III, IV and V respectively. Mice were sacrificed and mammary gland tissues were harvested after 24, 48, 72 and 96 h of infection. Body and mammary gland were weighed and histopathological responses of the tissue damage were observed for both dose and durational pathogenicity's of S. aureus inoculums.ResultsThe observations indicated mastitis symptoms, decrease in body and mammary gland weights. Histopathological evidences revealed massive infiltration of polymorphonuclear neutrophil leukocytes, damage of alveoli and secretory products as the doses and durations of S. aureus inoculum increased compared to the control.ConclusionThe present study revealed that damage in mammary gland tissue of mice increased as the dose and duration of S. aureus inoculums increased.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Disease - Volume 4, Supplement 1, 2014, Pages S320-S325