Occurrence of white spot syndrome virus in shrimp culturing waters and its brunt in specific pathogen free Litopenaeus vannamei with particular allusion to molecular verdicts

ObjectiveTo detect the water samples and shrimp samples in white spot syndrome virus (WSSV) affecting and non-affecting zone.MethodsA total of 12 samples specific pathogen free Litopenaeus vannamei (L. vannamei); adult shrimp and larvae were randomly collected. Their genomic DNA was isolated and subjected to PCR. Histopathological identifications were carried out, and the hematopoietic tissues with basophilic intranuclear inclusion bodies characteristic were observed in moderate WSSV infected L. vannameiResultsThe PCR analysis showed the appearance of a prominent band from the PCR amplified product of WSSV-DNA at internal control band 848 bp at non-infected areas. Although low infection positive bands (20 copies) were shown at 296 bp continued from initial stage of the infection region. On a moderate and ascetic level were observed as 650 bp and 910 bp (200 and 2 000 copies), during the severe out break periods. The gill epithelial cells were edematous and nuclei were hypertrophied with basophilic inclusions, but no pathological changes or hypertrophied nuclei were observed in any of L.vannamei tissues in WSSV uninfected region. The Intranuclear inclusion bodies characteristics of high level of WSSV infection presented in the gill region.ConclusionsThe present study is significant, which investigated the level of WSSV transmission from the infected tiger prawn P.monodon to SPFL. vannamei in the WSSV impact region of Tamil Nadu coastal waters.