Do diverse Mycobacterium tuberculosis strains circulate in Mumbai?

ObjectiveTo facilitate large network surveillance, we evaluated use of spoligotyping in combination with different formats (12-, 15- and 24- loci) of to Mycobacterial Interspersed Repetitive Units (MIRU)-Variable Number Tandem Repeats (VNTR) achieve maximal discriminatory power.MethodsSpoligotyping and 24-loci MIRU-VNTR was performed on 127 consecutive extrapulmonary Mycobacterium tuberculosis (M. tuberculosis) isolates.ResultsBased on the analysis of 127 M. tuberculosis isolates, all 3 formats of MIRU-VNTR when coupled with spoligotyping (HGDI: spoligotyping +12-loci, 0.9967