Effect and evaluation of antihyperlipidemic activity guided isolated fraction from total methanol extract of Bauhinia variegata (linn.) in Triton WR–1339 induced hyperlipidemic rats

ObjectiveTo investigate the effect and evaluation of Anti-hyperlipidemic activity guided sub-fraction isolated from total methanolic extract of Bauhinia variegata (Linn.) leaves on Triton WR–1339 induced hyperlipidemic rats.MethodsColumn chromatographic fractionation of butanol fraction of total methanol extract of leaves of Bauhinia variegata (Linn.) yields four sub-fractions (sub-fraction A-D). All sub-fractions tested for their anti-hyperlipidemic activity. Sub-fractions administered at a dose of 65 mg/kg (oral) to the Triton WR–1339 induced hyperlipidemic rats and total cholesterol, triglycerides, HDL, LDL and VLDL level in the blood were checked.ResultsSub-fraction D showed significant reduction (P<0.05) among four sub-fraction in comparison with standard drug fenofibrate.ConclusionsFrom the above study it could be concluded that butanol sub-fraction D of Bauhinia variegata (Linn.) not only have resulted in significant reduction in cholesterol, triglyceride, LDL, VLDL level but also increases the HDL level at a reduced dose level.