کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3459769 1231188 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of Intracoronary Infusion of Bone Marrow Mononuclear Cells or Peripheral Endothelial Progenitor Cells on Myocardial Ischemia-reperfusion Injury in Mini-swine
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Effect of Intracoronary Infusion of Bone Marrow Mononuclear Cells or Peripheral Endothelial Progenitor Cells on Myocardial Ischemia-reperfusion Injury in Mini-swine
چکیده انگلیسی

ObjectiveTo simulate and assess the clinical effect of intracoronary infusion of bone marrow mononuclear cells or peripheral endothelial progenitor cells on myocardial reperfusion injury in mini-swine model.MethodsTwenty-three mini-swine with myocardial reperfusion injury were used as designed in the study protocol. About (3.54±0.90)×108 bone marrow mononuclear cells (MNC group, n=9) or (1.16± 1.07)×107 endothelial progenitor cells (EPC group, n=7) was infused into the affected coronary segment of the swine. The other mini-swine were infused with phosphate buffered saline as control (n=7). Echocardio-graphy and hemodynamic studies were performed before and 4 weeks after cell infusion. Myocardium infarction size was calculated. Stem cell differentiation was analyzed under a transmission electromicroscope.ResultsLeft ventricular ejection fraction dropped by 0% in EPC group, 2% in MNC group, and 10% in the control group 4 weeks after cell infusion, respectively (P<0.05). The systolic parameters increased in MNC and EPC groups but decreased in the control group. However, the diastolic parameters demonstrated no significant change in the three groups (P<0.05). EPC decreased total infarction size more than MNC did (1.60±0.26 cm2vs. 3.71±1.38 cm2, P<0.05). Undermature endothelial cells and myocytes were found under transmission electromicroscope.ConclusionsTransplantation of either MNC or EPC may be beneficial to cardiac systolic function, but might not has obvious effect on diastolic function. Intracoronary infusion of EPC might be better than MNC in controlling infarction size. Both MNC and EPC may stimulate angiogenesis, inhibit fibrogenesis, and differentiate into myocardial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Medical Sciences Journal - Volume 25, Issue 3, September 2010, Pages 176-181