Development of bioanalytical parameters for standardization of Terminalia arjuna

ObjectiveTo develop a noval bioanalytical parameters for standardization of Terminalia arjuna (T. arjuna) extract.MethodsIn the present investigation, parameters such as preliminary phytochemical analysis, total phenol, flavonoid, tannin content, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis by HPTLC method were performed in order to standardization.ResultsPreliminary phytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, saponin, flavonoid, amino acid and protein. Loss on drying and solubily in water was found to be 5.04% and 81.10%. Total flavonoid and phenol content was found to be 10.4% and 0.44%. Total tannin content was found to be 24.8%. The content of quercetin and rutin in T. arjuna was found to be 1.08%w/w and 0.16%w/w respectively. Further the level of heavy metal and microrganism were found to be under the limit.ConclusionsThese bioanalytical parameters can be used as an important tool for the food scientists, researchers and even the consumers for its standards.