Determination of bioanalytical parameters for the standardization of Abroma augusta

ObjectiveTo set the analytical parameters for simultaneous determination of different phytochemicals present in the Abroma augusta (A. augusta).MethodsDifferent phytochemical test including total phenol and total flavonoid content were carried out in the present investigation. Further, a rapid and simple high-performance thin-layer chromatographic (HPTLC) method was developed for the qualitative and quantitative analysis. Moreover antimicrobial study was also performed in the present investigation to know the presence of biological contamination.ResultsPreliminary phytochemical analysis revealed the presence of glycoside, alkaloid, carbohydrate and steroid in the A. augusta. Total phenol and total flavonoid content were found to be 0.29% and 0.50% w/w respectively. All tested microorganism in the A. augusta extract were found to be under the limit. Chloroform: methanol: acetic acid: H2O (170:25:25:4) was used as a solvent system for the qualitative and quantitative analysis through HPTLC Method. HPTLC analysis revealed 1.87 % w/w of linoleic acid in the A. augusta extract.ConclusionsResults showed that the selected parameters in the present investigation would set the new standards for the qualitative and quantitative analysis of A. augusta.