کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3477471 1233330 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of Purinergic Mechanism for the Treatment of Voiding Dysfunction: A Study in Conscious Spinal Cord-injured Rats
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Evaluation of Purinergic Mechanism for the Treatment of Voiding Dysfunction: A Study in Conscious Spinal Cord-injured Rats
چکیده انگلیسی

BackgroundTo investigate the effect of a selective P2X3–P2X2/3 purinergic receptor antagonist (A-317491) on detrusor hyperreflexia in conscious chronic spinal cord-injured female rats.MethodsSix chronic spinal cord-transected female Sprague-Dawley rats (290–336 g) were used in this study. Spinal transection at the T8–T9 segmental level was performed using aseptic techniques under halothane anesthesia. Fourteen to 16 weeks after spinal transection, A-317491, a selective P2X3 purinergic receptor antagonist, was administered intravenously in cystometry studies at increasing doses of 0.03, 0.1, 0.3, 1, 3, 10 and 30 mmol/kg at 40–50 minute intervals. Cystometrograms (CMGs) were performed before and after the administration of each dose of the drug.ResultsThe continuous filling of CMGs revealed a large number of small-amplitude (> 8 cmH2O), non-voiding contractions (NVCs) (average, 9.7 per voiding cycle) preceding voiding contractions (mean amplitude, 31 cmH2O; duration, 2.5 minutes), which occurred at an interval of 539 seconds and at a pressure threshold of 5.7 cmH2O. When tested in a range of doses (0.03–30 μmol/kg, intravenous), A-317491 in doses between 1 and 30 mmol/kg significantly (p < 0.05) increased the interval between voids by 25%, reduced the number of NVCs by 42–62%, and increased the pressure threshold for voiding by 53–73%, but did not change the amplitude of the duration of the voiding contractions. The effects of the drug were apparent within 10 minutes following administration.ConclusionThese results indicate that purinergic mechanisms, presumably involving P2X3 or P2X2/3 receptors on bladder C-fiber afferent nerves, play an important role in the detrusor hyperreflexia that occurs after spinal cord injury in rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Chinese Medical Association - Volume 70, Issue 10, October 2007, Pages 439-444