کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3478673 1233409 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cyclosporine A induces connective tissue growth factor expression in human gingival fibroblasts: Suppression by epigallocatechin-3-gallate
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Cyclosporine A induces connective tissue growth factor expression in human gingival fibroblasts: Suppression by epigallocatechin-3-gallate
چکیده انگلیسی

Background/PurposeTransforming growth factor-β (TGF-β) plays an important role in the pathogenesis of cyclosporine A (CsA)-induced gingival overgrowth (GO). Connective tissue growth factor (CTGF/CCN2) acts as a cofactor with TGF-β to induce the maximal profibrotic effects of TGF-β. We investigated the effects of CsA on CCN2 expression in human gingival fibroblasts (HGFs) and the potential chemopreventive agent for CsA-induced GO.MethodsWestern blot analyses were used to examine the signaling pathways of CsA-induced CCN2 expression in HGFs and whether epigallocatechin-3-gallate (EGCG), curcumin, or lovastatin can inhibit CsA-induced CCN2 expression.ResultsCsA significantly stimulated CCN2 synthesis in HGFs. This effect can be inhibited by c-Jun NH2-terminal kinase (JNK) and Smad3 inhibitors but not by TGF-β neutralizing antibody and TGF-β type I receptor inhibitor. Furthermore, EGCG completely blocked CsA-induced CCN2 expression.ConclusionCsA-induced CCN2 protein expression is mediated through JNK and Smad signaling. CsA may contribute to the pathogenesis of GO through upregulation of CCN2 expression in HGFs. EGCG could be an adjuvant for the prevention of CsA-induced GO.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Formosan Medical Association - Volume 113, Issue 11, November 2014, Pages 828–832
نویسندگان
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