کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3482375 1596798 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The expressions of brain-derived neurotrophic factor and nerve growth factor in purified rat choroid plexus epithelial cells in vitro
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
The expressions of brain-derived neurotrophic factor and nerve growth factor in purified rat choroid plexus epithelial cells in vitro
چکیده انگلیسی

ObjectiveTo detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro.MethodsPrimary and passage choroid plexus epithelial cells were obtained from newborn, one-day Sprague-Dawley rats. The expressions of BDNF and NGF were measured by qRT-PCR and Western blottingting. The secretions of BDNF and NGF were detected by ELISA. Cell supernatants of primary cells, purified cells and passage 1 cells were harvested.ResultsThe expression of BDNF in the purified cells was significantly lower than that in the primary cells (P<0.05), and it in the primary cells and the purified cells was significantly higher than that in the passage 1 cells (P<0.05). The expression of NGF was significantly higher in the purified cells than in the primary cells and the passage 1 cells (P<0.05). It in the passage 1 cells was significantly higher than that in the primary cells (P<0.05).ConclusionThe time of CPECs transplantation for central nervous system diseases should be selected based on their secretory function and features, which could lead to better and more effective treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Medical Colleges of PLA - Volume 28, Issue 5, October 2013, Pages 257-267