Low polar volatile constituents from Isodon excisa by GC-MS

An attempt had been made to isolate and identify the low polar volatile constituents from Isodon excisa. The dried plants were heat extracted with ethanol and then extracted with petroleum ether. The constituents were isolated and analyzed by GC-MS and then we retrieved them on NIST08 mass spectrometry database to determine their structures. Finally, successful results were achieved, 111 compounds were obtained and identified. 105 of them were reported for the first time in Isodon excisa and 22 of them were first found in natural products. Thus, the main constituents of it were found to be Sitosterol (33.28%), 3-Amino-4-pyrazolecarbonitrile (9.55%).