Genetic imprinted gene PEG10 expression in deciduas of normal early pregnant women

ObjectiveTo investigate the role and significance of paternally expressed gene 10 (PEG10) in deciduas of normal pregnant women.MethodsSixty deciduas tissue from pregnant 6 to 11 weeks were divided into six groups and in each group ten samples were done every gestational week. The expression and distribution of PEG10 in deciduas were examined by RT-PCR, hybridization in situ, Western Blot and laser scanning confocal microscopy.ResultsThe PEG10 mRNA and protein were expressed in deciduas tissue from pregnant 6 to 11 weeks. Among them, the expression of PEG10 showed a gradual increase as the pregnancy weeks increased. In RT-PCR, the PEG10 expression was lower at pregnant 6th week (0.6743±0.114), from pregnant 7 to 8 weeks, the expression was increased gradually (7th week 0.7349±0.0083) and reached the pinnacle at 8th week (0.7354±0.0074). And then the pinnacle began to descend (9th week 0.6340±0.0084, 10th week 0.5901±0.0089 and 11th week 0.5261±0.0112). There was a significant difference in the expression of PEG10 from pregnant 6 to 11 weeks except 7th week and 8th week. This expression characteristic was demonstrated by hybridization in situ. The similar conclusion was proved by Western Blot and laser scanning confocal microscopy.ConclusionExpression of PEG10 may play an important role in early pregnancy.