A novel vWF A3-GPI modified EPCs to enhance its adhesion ability to collegen

ObjectiveTo gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro.MethodsThe plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM.ResultsFusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction.ConclusionThese findings represent a novel strategy in EPC transplantation treatment for vessel injury.