Related gene expressions in anti-keratinocyte aging induced by Ganoderma lucidum polysaccharides⋆

ObjectiveTo examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.MethodsHacaT cell of keratinocytes lines were cultured and treated with the polysaccharides. The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription. The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip. The images were scanned and analyzed with special software. The scan data were analyzed with software and checked by real time PCR.ResultsAmong total 18 346 human genes, the expression of 103 ones was up-regulated and 378 ones down-regulated. It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging. Two ways are anastomotic.ConclusionIt is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging. It shows that the results of gene array reliable by real time PCR.